Current practice of using biophysical techniques to assess postmortem interval and mechanical injuries: a review

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Abstract

The article analyzes the biophysical techniques currently used in forensic medicine to determine the post-mortem interval and identify characteristic changes in the tissues and organs of corpses. The review covers a wide range of methods, including thermophysical, optical, and impedance-based approaches, as well as advanced technologies such as mathematical modeling of algor mortis processes and tissue injury assessment.

The article emphasizes the importance of integrating biophysical techniques into forensic practices to improve diagnostic precision and investigative outcomes. This review combines theoretical and experimental approaches to provide more profound insights into the underlying mechanisms of the discussed techniques. This work is relevant because of the need to integrate new technologies and techniques into forensic practices and to optimize existing approaches.

The authors emphasize the significance of an interdisciplinary data analysis to facilitate more accurate post-mortem interval estimation and better characterization of mechanical injuries, offering great potential for future research in this field.

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Gulgena R. Mustafina

Bashkir State Medical University

Email: gulgenarm@mail.ru
ORCID iD: 0000-0003-2534-6385
SPIN-code: 8904-2046

MD, Cand. Sci. (Medicine) Assistant Professor

Russian Federation, Ufa

Airat A. Khalikov

Bashkir State Medical University

Email: airat.expert@mail.ru
ORCID iD: 0000-0003-1045-5677
SPIN-code: 1895-7300

MD, Dr. Sci. (Medicine), Professor

Russian Federation, Ufa

Kirill O. Kuznetsov

Bashkir State Medical University

Author for correspondence.
Email: kuznetsovarticles@mail.ru
ORCID iD: 0000-0002-2405-1801
SPIN-code: 3053-3773

MD

Russian Federation, Ufa

References

  1. Kil’dyushov EM, Ermakova YuV, Tumanov EV, Kuznetsova GS. Estimation of time since death in the late postmortem period in forensic medicine (literature review). Russian Journal of Forensic Medicine. 2018;4(1):34–38. doi: 10.19048/2411-8729-2018-4-1-34-38 EDN: YWDARF
  2. Khalikov AA, Kildyushov EM, Kuznetsov KO, Rahmatullina GR. Estimation of time since death with the postmortem microbiome: a modern view and approaches to solving the problem. Forensic Medical Expertise. 2022;65(3):49–53. doi: 10.17116/sudmed20226503149 EDN: TQGZHP
  3. Indiaminov SI, Zhumanov ZE, Blinova SA. Problems of establishing the prescription of death. Forensic Medical Expertise. 2020;63(6):45–53. doi: 10.17116/sudmed20206306145 EDN: FXLSCS
  4. Wu Z, Guo Y, Hayakawa M, et al. Artificial intelligence-driven microbiome data analysis for estimation of postmortem interval and crime location. Frontiers in Microbiology. 2024;15:1334703. doi: 10.3389/fmicb.2024.1334703 EDN: SAKUFJ
  5. Popov VL, Kazakova EL, Lavrukova OS, Polyakov AYu. On the prospects of the impedance monitoring method for determining the prescription of death coming. Forensic Medical Expertise. 2023;66(2):20–25. doi: 10.17116/sudmed20236602120 EDN: MQZICF
  6. Khalikov AA, Amineva GM, Kuznetsov KO, et al. Personality factors’ influence (gender, age, death category) on indicators of biophysical objectification of intra-vital injuries of a putrefactive corpse. Forensic Medical Expertise. 2021;64(4):25–29. doi: 10.17116/sudmed20216404125 EDN: YTMJFG
  7. Vavilov AYu, Rykunov IA. Determination of duration of fresh water exposure on corpse’s skin by dispersion value of its electrical conductivity. Forensic Medical Expertise. 2024;67(5):24–28. doi: 10.17116/sudmed20246705124 EDN: QBEDIG
  8. Khalikov AA, Kildyushov EM, Kuznetsov KO, et al. Diagnostics of the presence of once of death and peculiarities of performance of the forensic medical examination in post-mortal facial condition. Forensic Medical Expertise. 2022;65(2):16–19. doi: 10.17116/sudmed20226502116 EDN: JTQRDR
  9. Khalikov AA, Kanzafarova GA, Pozdeev AR, Kuznetsov KO. Analysis of optical density of pericardial liquid by means of pho-tocolorimetry as one of the methods for determining tanatogene-sis type. Modern Problems of Science and Education. 2021;(2):146. doi: 10.17513/spno.30656 EDN: IDYDIT
  10. Lepeshkin VV. Ultra-weak luminescence of biological objects. Priroda. 1934;(5):12–17. (In Russ.)
  11. Perelygin VV, Tarusov BN. Enhanced ultraweak radiation from injured tissues. Biophysics. 1966;11(3):539–541. EDN: SMRKZR
  12. Baskakov VG. Method of electrobio-luminescence spectroscopy as part of complex biophysical studies of the postmortem interval. Forensic Examination and Criminalistics Issues. 1997;(6):65–68. (In Russ.)
  13. Prutovykh VV. Expertise in establishing the vitality and duration of mechanical injury based on certain biophysical and biochemical parameters of skeletal muscles: [dissertation abstract]. Moscow, 1978. 20 p. (In Russ.) Available from: https://rusneb.ru/catalog/000200_000018_rc_2266631/
  14. Ananyev GV. On the possibility of diagnosing the age of bruises using chemiluminescence. In: Modern laboratory methods for determining the age of processes and objects of forensic examination. Moscow: Moscow State Medical Institute named after N.I. Pirogov; 1982. P. 48–50. (In Russ.)
  15. Azhipa YaI. Medico-biological aspects of applying the method of electron paramagnetic resonance. Moscow: Nauka; 1983. (In Russ.) Available from: https://search.rsl.ru/ru/record/01001165845
  16. Oganesyan MA. Determination of the age of mechanical trauma based on cytological, histochemical, and biophysical indicators of blood: [dissertation]. Yerevan; 1984. 218 p. (In Russ.) Available from: https://elib.nlb.by/catalog/Record/BY-NLB-rr17117630000/
  17. Buttaeva GM. Expert diagnosis of the vitality of vascular injuries in the late postmortem period (using the method of paramagnetic resonance): [dissertation abstract]. Moscow; 1986. 24 p. (In Russ.)
  18. Proshutin VL. Expert diagnosis of the vitality of mechanical trauma based on EPR spectra parameters of bone marrow: [dissertation abstract]. Moscow; 1988. 20 p. (In Russ.) EDN: ZSHYKZ
  19. Aushev RA. Determination of the vitality of fractures based on the state of the periosteal microcirculation and the spin probe recovery reaction of muscle tissue: [dissertation abstract]. Moscow; 1990. 24 p. (In Russ.) Available from: https://search.rsl.ru/ru/record/01000127027
  20. Kryukov VN, Siryatsky AA. Differential diagnosis of the vitality of blunt trauma depending on timing and cause of death. In: Collection of scientific papers Forensic medical determination of the mechanism, vitality, sequence and duration of mechanical damage. Moscow: 2nd MOLGMI; 1983. P. 49–53. (In Russ.)
  21. Tenkov AA, Tumanova NA. Dynamics of dielectric indicators of intact and injured soft tissues in the postmortem period. Forensic Medical Expertise. 1982;(2):12–13. (In Russ.)
  22. Lubychnikova PA, Khanadeyev VA. Some aspects of the application of biophysical methods in forensic medicine. In: Collection of articles from the VII International Research Competition “Best Research Article 2023”. Petrozavodsk: International Center for Scientific Partnership “New Science” 2024:60–65. EDN: NFTAIG
  23. Novoselov VP, Savchenko SV, Sakovchuk OA, Stepakov DA. Perspectives on the study of the eye globe environment in forensic diagnosis of time since death. In: Proceedings of the scientific and practical conference “Forensic Medicine: Issues, Problems, Expert Practice”. Novosibirsk: STT Limited Liability Company; 2020. P. 107-111. (In Russ.) EDN: EEYUIH
  24. Frolova OO, Zabozlaev FG, Klevno VA. Use of various research methods in forensic practice to determine lifetime and age of injury: A review. Russian Journal of Forensic Medicine. 2023;9(2):147–163. doi: 10.17816/fm6696 EDN: ZMZIGV
  25. Lakhno AV, Khalikov AA, Naidenova TV, Babushkina KA. Mathematical modeling of postmortem cooling of a corpse at unstable external temperatures. Modern Problems of Science and Education. 2019;(1):23–23. EDN: YYHRDF
  26. Nedugov GV. Estimation of the postmortem interval by the method of finite element modeling of postmortem heat transfer in human head. Science and Innovations in Medicine. 2022;7(3):179–185. doi: 10.35693/2500-1388-2022-7-3-179-185 EDN: CIMZZD
  27. Nedugov GV. Mathematical estimation of errors in determining the prescription of death by cranioencephalic temperature. Bulletin of Forensic Medicine. 2022;(1):9–13. EDN: OQAJIQ
  28. Nedugov GV. Mathematical modeling of the corpse cooling under conditions of varying ambient temperature. Russian Journal of Forensic Medicine. 2021;7(1):29–35. doi: 10.17816/fm360 EDN: SEWFID
  29. Vavilov AY, Khalikov AA, Rykunov IA, et al. Determination of the corpse’s stay in the water duration according to maceration degree of its skin. Forensic Medical Expertise. 2023;66(3):64–68. doi: 10.17116/sudmed20236603164 EDN: BOUGOE
  30. Agzakhodjaev TS, Rasulova XA, Shorakhmedov ShSh, Ortiqboyev JO. Diagnostic advantages of contactless bioimpedanesometry. New Day in Medicene. 2020;(2):293–300. EDN: BSNHNI
  31. Bachynsky VT, Vanchuliak OYa, Garazdiuk MS. Investigation of cerebrospinal fluid in the forensic practice for determin the time of death. Bulletin of Forensic Medicine. 2015;4:8–11. EDN: VPIHZX
  32. Pligin AV, Kislov MA, Maksimov AV, Klevno VA. The problems of determining of the age of bruises by modern diagnostic methods. Literature review. Russian Journal of Forensic Medicine. 2022;8(1):51–58. doi: 10.17816/fm699 EDN: DOPKVV
  33. Lavrukova OS, Kazakova EL, Polyakov AYu. Postmortem tissue changes and dynamics of their impedance parameters: a preclinical experimental study. Kuban Scientific Medical Bulletin. 2023;30(5):77–86. doi: 10.25207/1608-6228-2023-30-5-77-86 EDN: KIEPLW
  34. Naydenova TV, Babushkina KA. Colorimetric methods of instrumental analysis in forensic medicine (scientific review). Eurasian Scientific Union. 2018;12(3):170–174. (In Russ.) EDN: YTUCAP
  35. Vavilov AYu, Lakhno AV. Determination of the presence of human death by the degree of restoration of the corpse spot by the photocolorimetric method. Modern Problems of Science and Education. 2021;(3):164. doi: 10.17513/spno.30921 EDN: FUEHZY
  36. Sidorov VL, Loban IE, Gusarov AA, et al. Determination the presence of saliva in traces on material evidence by α-amilase content using the colorimetry method. Bulletin of Forensic Medicine. 2020;(3):17–22. EDN: EGQWFL
  37. Fominykh TA, Kutsevol BL, Saenko AG, Gritskevich OYu. Basic research methods in modern forensic medicine. Krymskii zhurnal eksperimental'noi i klinicheskoi meditsiny. 2021;(4):106–117. doi: 10.37279/2224-6444-2021-11-4-106-117 EDN: FDWDEE
  38. Mazur ES, Ivanov IV. Use of special knowledge in investigation of crimes. Russian Journal of Criminal Law. 2021;(7):121–126. doi: 10.17223/23088451/7/19
  39. Kildyushov EM, Egorova EV, Burenchev DV. Modern capabilities of radiologic imaging in forensic medicine. Russian Journal of Forensic Medicine. 2019;5(4):4–8. doi: 10.19048/2411-8729-2019-5-4-4-8 EDN: WUEIXZ
  40. Kokov LS, Kinle AF, Sinitsyn VE, Filimonov BA. Possibilities of computed tomography and magnetic resonance imaging in forensic medical examination of mechanical trauma and sudden death (a literature review). Zhurnal im N V Sklifosovskogo Neotlozhnaya meditsinskaya pomoshch’. 2015;(2):16–26. EDN: TYJOVR
  41. Dirnhofer R, Jackowski C, Vock P, et al. VIRTOPSY: Minimally Invasive, Imaging-guided Virtual Autopsy. RadioGraphics. 2006;26(5):1305–1333. doi: 10.1148/rg.265065001
  42. Joseph TI, Girish KL, Sathyan P, et al. Virtopsy: An integration of forensic science and imageology. J Forensic Dent Sci. 2017;9(3):111–114. doi: 10.4103/jfo.jfds_52_16
  43. Staicu A, Albu C, Popa-Stanila R, et al. Whole-body non-forensic fetal virtopsy using postmortem magnetic resonance imaging at 7 Tesla vs classical autopsy. Ultrasound in Obstetrics & Gynecology. 2024;64(5):661–668. doi: 10.1002/uog.29106 EDN: ADYWMU
  44. Klevno VA, Chumakova YuV. Virtopsy – new method of research in national practice of forensic medicine. Russian Journal of Forensic Medicine. 2019;5(2):27–31. doi: 10.19048/2411-8729-2019-5-2-27-31 EDN: JPKOKY
  45. Klevno VA, Chumakova YuV, Kurdyukov FN, et al. Possibilities Of Posthumous Computer Tomography (Virtual Autopsy) In The Event Of Death From Mechanical Asphixia. Russian Journal of Forensic Medicine. 2018;4(4):22–26. doi: 10.19048/2411-8729-2018-4-4-22-26 EDN: PMIIDQ
  46. Eremchenko VI. Prospects for the use of modern instrumental methods of research in the production of forensic examination of the corpse. Bulletin of Krasnodar University of Russian MIA. 2019;(3):65–69. EDN: XFJLYD
  47. Erofeev SV, Shishkin YuYu, Fedorova AS. About the technology of image analysis as a means of increasing the objectivity and reliability of forensic examinations. Russian Journal of Forensic Medicine. 2017;3(2):17–23. doi: 10.19048/2411-8729-2017-3-2-17-23 EDN: YZLKHR
  48. Strelkov AA, Dadabaev VK. Preliminary forensic examination of a corpse using computed tomography: possibilities and prospects. Humanities and Law Research. 2014;(3):122–126. EDN: TSZVTR
  49. Kliplko EP. Method For Determining The Cause Of Death When Any Written Statement Is Obtaining To Refuse From Possible Explosion. Vestnik Eastern Siberia Institute of the Ministry of the Interior of the Russian Federation. 2018;87:190–196. EDN: YQXMWD
  50. Tumanova UN, Uslontsev DN, Krupnov NM, et al. Determining the time of death of newborns and infants using postmortem magnetic resonance imaging of eye structures. In: Collection of scientific papers of the medical faculty of KRSU Problems and challenges of fundamental and clinical medicine in the XXI century “The current state and prospects for the development of forensic medicine and morphology in the context of the formation of the Eurasian Economic Union”. Bishkek: Kyrgyz-Russian Slavic University; 2020. P. 110–120. EDN: RKSGGF
  51. Klevno VA, Chumakova YuV, Kurdyukov FN, et al. Virtopsy of the body of a girl-adolescent died after fall from a great height. Russian Journal of Forensic Medicine. 2019;5(1):11–15. doi: 10.19048/2411-8729-2019-5-1-11-15 EDN: AJBBQJ
  52. Tumanova UN, Shchegolev AI, Kovalev AV. Organization of postmortem radiological examination in the structure of pathological departments and forensic bureaus. Forensic Medical Expertise. 2021;64(1):57–63. doi: 10.17116/sudmed20216401157 EDN: HIAXNU
  53. Prikhod’ko AN, Lavrukova OS. On biological objects’ usage for the postmortem interval determination. Proceedings of Petrozavodsk State University. 2017;(2):85–88. EDN: YHOKAJ
  54. Tenkov AA, Plaksin VO. Forensic medical examination of a corpse in the late post-mortem period (decay, adipocere, mummification, assessment of injuries). Kursk; 2005. ISBN: 5-7487-0932-5 (In Russ.) EDN: QLKVZL
  55. Dmitrienko YuA. Change in viability of nucleated bone marrow cells. Sovremennaya diagnostika v sudebnoi meditsine. 1981:41–43. (In Russ.)
  56. Bunin YuN. Changes in the active electrical resistance of cadaver skin depending on the postmortem interval (experimental study): [dissertation abstract]. Barnaul; 1978. 40 p. (In Russ.)
  57. Korovin AA. Comprehensive assessment of morphological and biophysical changes in the tissues and organs of corpses in forensic medical diagnosis of the postmortem interval: [dissertation abstract]. Moscow; 2000. 41 p. (In Russ.)
  58. Pigolkin YuI, Korovin AA. Dependence of autolysis processes on ambient temperature according to impedance plethysmography. Aktual'nye aspekty sudebnoi meditsiny. 1999;(5):116–118. (In Russ.)
  59. Nikiforov YaA. Determination of the postmortem interval by changes in the electrical resistance of kidneys and Achilles tendons: [dissertation abstract]. Izhevsk; 2003. 24 p. (In Russ.) Available from: https://medical-diss.com/docreader/224883/d#?page=1
  60. Onyanov AM. Dynamics of impedance indicators of the vitreous body in the late postmortem period: [dissertation abstract]. Izhevsk; 2008. 24 p. (In Russ.) EDN: NJESAP
  61. Chirkov VE, Vavilov AYu. Impedance as a diagnostic criterion for vitality and age of blood traces. In: Proceedings of the International Scientific and Practical Conference “Current Issues in Forensic Science and Forensic Expertise”. Irkutsk: East Siberian institute of the Ministry of Internal Affairs of the Russian Federation; 2008. P. 76–79. (In Russ.) Available from: https://studfile.net/preview/21495698/page:8/
  62. Nikolaev BS. Determining the time from the onset of bleeding into the pleural and abdominal cavities to cardiac arrest. In: Proceedings of the II All-Russian Congress of Forensic Physicians: abstracts of reports. Irkutsk-Moscow; 1987. P. 271–272. (In Russ.)
  63. Viter VI, Surkov YuG, Pozdeev AR, et al. The estimation of craniocerebral trauma's heaviness by spectrum specific electroconductivity'sofcerebrospinalfluidinearlypostmortalperiod. Medical Examination Problem. 2004;3(15):12–14. (In Russ.) EDN: OKGUTZ
  64. Saakyan ES. Parameters of electrical conductivity, morphological and histological changes of skeletal muscles in the dynamics of the post-traumatic period: [dissertation abstract]. Moscow; 1992. 21 p. (In Russ.)
  65. Kovaleva MS. Determination of the age of bruises by impedanceometry: [dissertation abstract]. Izhevsk; 2006. 24 p. (In Russ.) EDN: NIXTQP
  66. Patent RUS No. 2328737/ 10.07.2008. Byul. No. 19. Khalikov AA, Viter VI, Vavilov AYu. Analysis of bruise prescription on cadaver. (In Russ.) Available from: https://patentimages.storage.googleapis.com/f9/03/62/04ca764726c36b/RU2328737C1.pdf
  67. Khalikov AA, Viter VI. The significance of biophysical studies in the diagnosis of the age of mechanical trauma. Meditsinskaya ekspertiza i pravo. 2011;(6):38–41. (In Russ.) EDN: OKIFSJ
  68. Markelova NG. Comprehensive biophysical diagnosis of the age of bruises in living persons: [dissertation abstract]. Moscow; 2008. 24 p. (In Russ.) EDN: NKPAXN
  69. Litvinova YuN. Some morphological and biophysical changes in the walls of main vessels depending on the timing of their injury. Voprosy sudebnoi meditsiny i ekspertnoi praktiki. 1973;(5):309–311. (In Russ.)
  70. Tomilina LA. Biophysical changes in soft tissues as forensic criteria for determining the age and vitality of injuries in the examination of a dismembered corpse: [dissertation]. Barnaul; 1979. 157 p. (In Russ.) Available from: https://search.rsl.ru/ru/record/01007760859
  71. Zorkin AI. Forensic determination of the postmortem interval using biophysical studies of cadaver tissues: [dissertation abstract]. Barnaul; 1975. 19 p. (In Russ.) Available from: https://search.rsl.ru/ru/record/01006925977
  72. Ermakova YuV. Method for determining the postmortem interval using spin probes. Meditsinskaya ekspertiza i parvo. 2012;(1):32–34. (In Russ.) EDN: OXWMCV
  73. Buromsky IV. Dynamics of nucleic acid decay as a criterion for forensic determination of the postmortem interval [dissertation abstract]. Moscow; 1980. 22 p. (In Russ.)
  74. Dmitrenko YuA. Dynamics of bone marrow changes as a criterion for determining the postmortem interval (morphological, biochemical, and biophysical studies): [dissertation abstract]. Moscow; 1984. 23 p. (In Russ.) Available from: https://rusneb.ru/catalog/000200_000018_rc_790346/
  75. Avakyan NM, Kazaryan RS, Saakyan ES. Application of circular dichroism and spectrophotometry methods for determining the postmortem interval. Forensic Medical Expertise. 1989;32(4):6–8. (In Russ.)
  76. Knight B, Lauder I. Practical method of dating skeletal remains. Med Sci Law. 1967;7(4):205–208. doi: 10.1177/002580246700700408

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